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30 Difficult NEET-Level MCQs from NCERT Class 12 Biology Chapter 2: Human Reproduction

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Preparing for NEET 2025? Mastering the chapter Human Reproduction from NCERT Class 12 Biology is crucial for scoring high in the reproductive biology section. Here are 30 NEET practice MCQs strictly based on NCERT content, crafted with difficult and analytical options to simulate the actual NEET experience.


📘 Chapter 2: Human Reproduction – NEET Practice Questions


1. Which of the following statements regarding the human female reproductive system is not correct as per NCERT?

A. The uterus opens into the vagina through the cervix
B. The oviduct is divided into three regions: infundibulum, isthmus, and fimbriae
C. The inner glandular layer of the uterus is called endometrium
D. Fallopian tubes extend from the periphery of the ovaries to the uterus

Answer: B
Explanation: As per NCERT, the oviduct (fallopian tube) is divided into infundibulum, ampulla, and isthmus.


2. The correct chronological order of events during oogenesis is:

A. Multiplication → Growth → Maturation → Ovulation
B. Maturation → Multiplication → Growth → Ovulation
C. Multiplication → Maturation → Growth → Ovulation
D. Growth → Multiplication → Ovulation → Maturation

Answer: A


3. The acrosome of the sperm is derived from:

A. Golgi apparatus
B. Endoplasmic reticulum
C. Mitochondria
D. Centriole

Answer: A


4. The LH surge during the menstrual cycle is directly responsible for:

A. Thickening of endometrium
B. Rupture of Graafian follicle
C. Conversion of primary to secondary oocyte
D. Implantation of embryo

Answer: B


5. Which hormone is absolutely essential for maintenance of endometrium during pregnancy?

A. FSH
B. Estrogen
C. Progesterone
D. LH

Answer: C


6. In human males, sperm formation starts at puberty due to increased secretion of:

A. Oxytocin
B. Testosterone
C. FSH
D. LH

Answer: B


7. The maximum number of primary follicles a female has is during:

A. Birth
B. Puberty
C. Fertile period
D. Menopause

Answer: A


8. The site of fertilization in human females is:

A. Cervix
B. Uterus
C. Ampullary-isthmic junction of the fallopian tube
D. Ovary

Answer: C


9. Capacitation of sperm occurs in:

A. Vagina
B. Uterus
C. Epididymis
D. Female reproductive tract

Answer: D


10. Which of the following does not occur during the follicular phase?

A. Follicular growth
B. Regeneration of endometrium
C. LH surge
D. Formation of corpus luteum

Answer: D


11. Primary spermatocytes are:

A. Haploid
B. Diploid
C. Triploid
D. Polyploid

Answer: B


12. The function of Sertoli cells is to:

A. Secrete testosterone
B. Form the blood-testis barrier
C. Store mature sperm
D. Produce LH

Answer: B


13. The outermost layer of the blastocyst is:

A. Endometrium
B. Morula
C. Trophoblast
D. Zona pellucida

Answer: C


14. The enzyme responsible for digestion of the zona pellucida is:

A. Acrosin
B. Hyaluronidase
C. Trypsin
D. Pepsin

Answer: A


15. Which of the following statements is correct as per NCERT?

A. The secondary oocyte is released during ovulation
B. Spermatogenesis is completed at birth
C. Corpus luteum degenerates before ovulation
D. Fimbriae are connected to the uterus

Answer: A


16. The hormone that stimulates Leydig cells is:

A. FSH
B. LH
C. Progesterone
D. Estrogen

Answer: B


17. At what meiotic stage is the secondary oocyte arrested during ovulation?

A. Metaphase I
B. Anaphase I
C. Metaphase II
D. Prophase I

Answer: C


18. Which cells form the blood-testis barrier?

A. Leydig cells
B. Sertoli cells
C. Primary spermatocytes
D. Spermatogonia

Answer: B


19. What is the chromosomal condition of a secondary oocyte?

A. 2n
B. n
C. 4n
D. 3n

Answer: B


20. The organ in which implantation of the blastocyst occurs is:

A. Ovary
B. Cervix
C. Uterus
D. Fallopian tube

Answer: C


21. The release of GnRH occurs from:

A. Pituitary gland
B. Hypothalamus
C. Ovary
D. Testis

Answer: B


22. The structure that degenerates if fertilization does not occur:

A. Endometrium
B. Graafian follicle
C. Corpus luteum
D. Secondary oocyte

Answer: C


23. Which event marks the end of the luteal phase?

A. Ovulation
B. Menstruation
C. Fertilization
D. LH surge

Answer: B


24. First step in parturition is:

A. Delivery of fetus
B. Delivery of placenta
C. Dilation of cervix
D. Implantation

Answer: C


25. Which one is not a function of placenta?

A. Hormone secretion
B. Nutrition to embryo
C. Removal of waste
D. Initiation of cleavage

Answer: D


26. Colostrum is rich in:

A. Albumin
B. IgA
C. Fibrinogen
D. IgG

Answer: B


27. Human pregnancy is divided into:

A. 3 phases of 2 months
B. 2 phases of 5 months
C. 3 trimesters of 3 months
D. 4 trimesters of 2 months

Answer: C


28. Which hormone is not involved in milk ejection or synthesis?

A. Prolactin
B. Oxytocin
C. Estrogen
D. Progesterone

Answer: D


29. The zona pellucida is:

A. A germ layer
B. A membrane around the sperm
C. A glycoprotein layer around oocyte
D. A layer of uterus

Answer: C


30. Which of the following sequences is correct for spermatogenesis?

A. Spermatogonia → Primary spermatocyte → Spermatid → Sperm
B. Spermatid → Spermatogonia → Sperm → Secondary spermatocyte
C. Sperm → Spermatid → Spermatocyte → Spermatogonia
D. Spermatogonia → Spermatid → Primary spermatocyte → Sperm

Answer: A


These 30 difficult NEET MCQs from Chapter 2 – Human Reproduction are ideal for last-minute practice. Each question is crafted from NCERT lines, with NEET-style distractors to strengthen your conceptual clarity. Ensure you go through the NCERT textbook thoroughly, as nearly every question in NEET is conceptually rooted in it.


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